| Annotated protein: | Ephexin-1 (Eph-interacting exchange protein) (Neuronal guanine nucleotide exchange factor). Gene symbol: NGEF. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q8CHT1 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ NGEF |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of synapse disassembly (GO:1905806) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Fu WY, et al. "Cdk5 regulates EphA4-mediated dendritic spine retraction through an ephexin1-dependent mechanism" Nat Neurosci. 2007 Jan;10(1):67-76 PMID:17143272 |
| Figure(s): | Fig 6, 7, 8 |
| Annotation description: | Fig.6: "Ephexin1 is phosphorylated by Cdk5-p35" Fig.7: "Cdk5 phosphorylation of ephexin1 regulates EphA4-dependent GEF activity" Fig.8: "Cdk5-dependent phosphorylation of ephexin1 is required for the ephrin-A1-mediated spine retraction" - "In ephexin1 knockdown neurons, however, ephrin-A1 did not reduce spine density (Fig. 8a-c). Similarly, ephrin-A1 did not reduce spine density in neurons from genetically ephexin1-deficient (ephexin1-/-; Ngef1-/-) neurons (Fig. 8d,e)." |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons Non-neuronal tissue |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) Over-expression |
| Evidence tracking, Experiment Assay: | Confocal Western blot |
| Annotator(s): | Ryan J. Farrell (ORCID:0000-0003-4022-8707) Ghazaleh Ashrafi (ORCID:0000-0001-7480-0826) Camila Pulido (ORCID:0000-0002-5648-066X) Jaime de Juan-Sanz (ORCID:0000-0002-1212-5623) Timothy Ryan (ORCID:0000-0003-2533-9548) |
| Lab: | Department of Biochemistry, Weill Cornell Medicine, New York, NY 10065, USA |
| SynGO annotation ID: | 2450 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |