| Annotated protein: | Syntaxin-12 (Syntaxin-13). Gene symbol: STX12. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: G3V7P1 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ STX12 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of synaptic vesicle membrane (GO:0030285) |
| Synapse type(s): | hippocampus |
| Annotated paper: | Rizzoli SO, et al. "Evidence for early endosome-like fusion of recently endocytosed synaptic vesicles" Traffic. 2006 Sep;7(9):1163-76 PMID:17004320 |
| Figure(s): | Fig. 1 and 2 |
| Annotation description: | Costaining in neurons for the synaptic vesicle marker protein synaptophysin and syntaxin 13 shows significant colocalization for both proteins. This suggests that the early endosomal SNARE syntaxin 13 is localized to presynaptic compartments. Therefore, the authors perform subcellular fractionation of rat brain, followed by immunoblotting and immunoEM on purified SVs with antibodies against SV marker proteins and syntaxin 13. In line with the findings of Takamori et al (2006, Cell), syntaxin 13 shows enrichment in the SV fraction as compared to the other fractions. In addition, the immunoEM shows the presence of syntaxin 13 on a subset of the vesicles. |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Confocal Western blot Biochemical fractionation (generic) |
| Annotator(s): | Momchil Ninov (ORCID:0000-0002-0808-7003) Mahdokht Kohansalnodehi (ORCID:0000-0002-3898-5197) Reinhard Jahn (ORCID:0000-0003-1542-3498) |
| Lab: | Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, 37077 Göttingen, Germany |
| Additional literature: | Fig Suppl 3: Takamori et al demonstrate strong enrichment of syntaxin 13 in the highly purified synaptic vesicle fraction, which also contains synaptic early endosomes. @ PMID:17110340 |
| SynGO annotation ID: | 2440 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |