| Annotated protein: | Actin nucleation-promoting factor WASL (Neural Wiskott-Aldrich syndrome protein) (N-WASP). Gene symbol: WASL. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: O08816 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ WASL |
| Ontology domain: | Biological Process |
| SynGO term: | postsynaptic actin cytoskeleton organization (GO:0098974) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Wegner AM, et al. "N-wasp and the arp2/3 complex are critical regulators of actin in the development of dendritic spines and synapses" J Biol Chem. 2008 Jun 6;283(23):15912-20 PMID:18430734 |
| Figure(s): | Fig.2-4 |
| Annotation description: | Fig.2: "Inhibition of N-WASP activity by wiskostatin affects excitatory, but not inhibitory, synapses." - Literal: "wiskostatin treatment had a significant effect on the density of excitatory glutamatergic synapses as the number of PSD-95 puncta was decreased by >50% (Fig. 2C). Because excitatory synapses can form on dendritic spines or on the dendritic shaft, we assessed the effects of wiskostatin treatment on each of these types of excitatory synapses. Wiskostatin treatment decreased the number of excitatory synapses that formed on spines by almost 90% (20.4 ± 1.7 in controls versus 2.3 ± 0.6 in wiskostatin-treated) but had no significant effect on shaft synapses (20.2 ± 1.7 in controls versus 19.6 ± 1.6 in wiskostatin-treated)." Fig.3: "Knock down of endogenous N-WASP affects spines and synapses." - Literal: "When neurons were transfected with the N-WASP RNAi construct at day 6 and then fixed and stained for SV2 and PSD-95 at day 12, a 2- to 3-fold decrease in the number of dendritic spines and synapses was observed compared with cells expressing a scrambled RNAi construct or empty pSUPER vector (Fig. 3, B and C, and supplemental Fig. S2)." Fig.4: "N-WASP binding to actin and the Arp 2/3 complex is important for dendritic spine and synapse formation." - Literal: "Expression of GFP-N-WASP caused a small but significant increase in the density of dendritic spines and synapses compared with GFP alone (Fig. 4, B and C). Expression of N-WASP lacking the C-terminal VCA region (ΔWA) significantly decreased the number of spines and synapses compared with control neurons expressing GFP, but protrusions were still observed along the dendrites of N-WASP-ΔWA-expressing neurons (Fig. 4, B and C, and supplemental Fig. S3)." |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | RNAi / shRNA Over-expression Antagonist / agonist |
| Evidence tracking, Experiment Assay: | Wide-field fluorescence |
| Annotator(s): | Ryan J. Farrell (ORCID:0000-0003-4022-8707) Ghazaleh Ashrafi (ORCID:0000-0001-7480-0826) Camila Pulido (ORCID:0000-0002-5648-066X) Jaime de Juan-Sanz (ORCID:0000-0002-1212-5623) Timothy Ryan (ORCID:0000-0003-2533-9548) |
| Lab: | Department of Biochemistry, Weill Cornell Medicine, New York, NY 10065, USA |
| SynGO annotation ID: | 2438 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |