Annotated protein:Actin nucleation-promoting factor WASL (Neural Wiskott-Aldrich syndrome protein) (N-WASP). Gene symbol: WASL. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: O08816
antibody wiki:
SynGO gene info:SynGO data @ WASL
Ontology domain:Biological Process
SynGO term:postsynaptic actin cytoskeleton organization (GO:0098974)
Synapse type(s):hippocampus, glutamatergic
Annotated paper:Wegner AM, et al. "N-wasp and the arp2/3 complex are critical regulators of actin in the development of dendritic spines and synapses" J Biol Chem. 2008 Jun 6;283(23):15912-20 PMID:18430734
Figure(s):Fig.2-4
Annotation description:Fig.2: "Inhibition of N-WASP activity by wiskostatin affects excitatory, but not inhibitory, synapses."
- Literal: "wiskostatin treatment had a significant effect on the density of excitatory glutamatergic synapses as the number of PSD-95 puncta was decreased by >50% (Fig. 2C). Because excitatory synapses can form on dendritic spines or on the dendritic shaft, we assessed the effects of wiskostatin treatment on each of these types of excitatory synapses. Wiskostatin treatment decreased the number of excitatory synapses that formed on spines by almost 90% (20.4 ± 1.7 in controls versus 2.3 ± 0.6 in wiskostatin-treated) but had no significant effect on shaft synapses (20.2 ± 1.7 in controls versus 19.6 ± 1.6 in wiskostatin-treated)."

Fig.3: "Knock down of endogenous N-WASP affects spines and synapses."
- Literal: "When neurons were transfected with the N-WASP RNAi construct at day 6 and then fixed and stained for SV2 and PSD-95 at day 12, a 2- to 3-fold decrease in the number of dendritic spines and synapses was observed compared with cells expressing a scrambled RNAi construct or empty pSUPER vector (Fig. 3, B and C, and supplemental Fig. S2)."

Fig.4: "N-WASP binding to actin and the Arp 2/3 complex is important for dendritic spine and synapse formation."
- Literal: "Expression of GFP-N-WASP caused a small but significant increase in the density of dendritic spines and synapses compared with GFP alone (Fig. 4, B and C). Expression of N-WASP lacking the C-terminal VCA region (ΔWA) significantly decreased the number of spines and synapses compared with control neurons expressing GFP, but protrusions were still observed along the dendrites of N-WASP-ΔWA-expressing neurons (Fig. 4, B and C, and supplemental Fig. S3)."
Evidence tracking, Biological System:Cultured neurons
Evidence tracking, Protein Targeting:RNAi / shRNA
Over-expression
Antagonist / agonist
Evidence tracking, Experiment Assay:Wide-field fluorescence
Annotator(s):Ryan J. Farrell (ORCID:0000-0003-4022-8707)
Ghazaleh Ashrafi (ORCID:0000-0001-7480-0826)
Camila Pulido (ORCID:0000-0002-5648-066X)
Jaime de Juan-Sanz (ORCID:0000-0002-1212-5623)
Timothy Ryan (ORCID:0000-0003-2533-9548)
Lab:Department of Biochemistry, Weill Cornell Medicine, New York, NY 10065, USA
SynGO annotation ID:2438
Dataset release (version):20231201
View annotation as GO-CAM model:Gene Ontology