| Annotated protein: | Potassium voltage-gated channel subfamily H member 1 (Ether-a-go-go potassium channel 1) (EAG channel 1) (EAG1) (m-eag) (Voltage-gated potassium channel subunit Kv10.1). Gene symbol: KCNH1. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q60603 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ KCNH1 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of presynaptic cytosolic calcium levels (GO:0099509) |
| Synapse type(s): | Parallel fibre (cerebellum -> Purkinje-cell) |
| Annotated paper: | Mortensen LS, et al. "KV 10.1 opposes activity-dependent increase in Ca(2)(+) influx into the presynaptic terminal of the parallel fibre-Purkinje cell synapse" J Physiol. 2015 Jan 1;593(1):181-96 PMID:25556795 |
| Figure(s): | fig 1, 5, 6 |
| Annotation description: | 25556795 Fig 1, Voltage clamp experiments showing voltage-gated potassium-channel function. 25556795 Fig 6a-b,Increased paired pulse ratio (PPR) in KO. Biochemical process is inferred from: localisation of the channel and its general function, supported by changes in presynaptic function in the KO as a consequence of altered excitability. 6/2/2018 Pim - The in situ action of KCNH1 on membrane potential is not shown in the synapse itself. In stead, the authors show activity as VGPC in HEK cells (effects in situ on membrane potential could not be observed for reasons mentioned in the paper), show downstream effect on presynaptic calcium concentration (Fig.5) and SV exocytosis (Fig.6). Since the inference to 'voltage-gated ion channel activity involved in regulation of presynaptic membrane potential (GO:0099508)' is safely made, I approve of this annotation and created the secondary tems as well. |
| Evidence tracking, Biological System: | Intact tissue Non-neuronal tissue |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) Over-expression |
| Evidence tracking, Experiment Assay: | Whole-cell patch clamp Optical physiology |
| Annotator(s): | Niels Cornelisse (ORCID:0000-0001-9425-2935) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | Fig 3, Localisation in the presynaptic terminal Fig 5c, Increased Ca levels in presynaptic terminal after AP stimulation Fig 6a-b,Increased paired pulse ratio (PPR) in KO. @ PMID:25556795 Fig 1, Voltage clamp experiments showing voltage-gated potassium-channel function. @ PMID:10026145 |
| SynGO annotation ID: | 2429 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |