| Annotated protein: | Dystroglycan 1 (Dystroglycan) (Dystrophin-associated glycoprotein 1). Gene symbol: DAG1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: F1M8K0 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ DAG1 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic cytosol (GO:0099524) |
| Synapse type(s): | retina, glutamatergic |
| Annotated paper: | Ueda H, et al. "Beta-dystroglycan localization in the photoreceptor and Muller cells in the rat retina revealed by immunoelectron microscopy" J Histochem Cytochem. 1998 Feb;46(2):185-91 PMID:9446825 |
| Figure(s): | fig 2; fig 6 |
| Annotation description: | Fig 2; Confocal images of DAG1 distribution in the rat retina shows that DAG1 is expressed in synapses of cone cells. Literal: " In the obliquely cut sections, larger immunostaining dots were definitely recognized at higher magnifications (Figure 2C). Small dots may represent rod spherules and large ones cone pedicles. These findings were consistent with that of the dystrophin immunostaining pattern (Schmitz et al. 1993; Ueda et al. 1997). Therefore, b-DG has both synaptic and nonsynaptic localization." Fig 6; Immunolocalization of DAG1 in the cone cell membrane of the flat synapse. Literal: "Cone cells have two synapses, an invaginated synapse and a flat synapse (Figure 6). Invaginated synapses have horizontal cell processes and synaptic ribbons, but flat synapses have neither. Cone cell membranes facing the bipolar cell processes showed high electron density at both synapses as well as at the rod cell membrane. The inset to Figure 6 shows beta-DG immunoreactivity at a flat synapse under the cone cell membrane." |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Confocal |
| Annotator(s): | Chiara Verpelli (ORCID:0000-0003-2949-9725) Carlo Sala (ORCID:0000-0003-0662-9523) |
| Lab: | CNR Neuroscience Institute Milan and Dept. of Biotechnology and Translational Medicine, University of Milan, 20129 Milan, Italy |
| SynGO annotation ID: | 2411 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |