| Annotated protein: | Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (EC 1.2.1.12) (Peptidyl-cysteine S-nitrosylase GAPDH) (EC 2.6.99.-). Gene symbol: GAPDH. Taxonomy: Sus scrofa (Pig). Uniprot ID: P00355 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ GAPDH |
| Ontology domain: | Biological Process |
| SynGO term: | ATP metabolic process in the postsynapse |
| Synapse type(s): | neocortex, glutamatergic |
| Annotated paper: | Wu K, et al. "The synthesis of ATP by glycolytic enzymes in the postsynaptic density and the effect of endogenously generated nitric oxide" Proc Natl Acad Sci U S A. 1997 Nov 25;94(24):13273-8 PMID:9371836 |
| Figure(s): | Fig. 6 |
| Annotation description: | Results section: "Endogenous Formation of ATP in the PSD" - Literal: "...the PSD fractions were incubated with ADP, D,L-G3P, NAD, and 32Pi. The amount of ATP formed endogenously was determined by the Ca21yCaM-dependent phosphorylation of the PSD proteins (Fig. 6, lanes 1 and 2)." "By using the kinase activity to estimate the ATP amount, our results indicated that, in three independent experiments, the ATP formed endogenously was 369, 360, and 353 fmol ATP per mg PSD, resulting in an average yield of 361 6 2.1 fmol ATP per mg PSD protein. Endogenous formation of ATP with rat cortex PSD was similar to that using the porcine PSD (data not shown)." |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | none of the above |
| Evidence tracking, Experiment Assay: | Biochemical fractionation (generic) radioisotope assay evidence |
| Annotator(s): | Rita Reig-Viader (ORCID:0000-0002-6893-6177) Àlex Bayés (ORCID:0000-0002-5265-6306) |
| Lab: | Molecular Physiology of the Synapse Laboratory, Biomedical Research Institute Sant Pau, 08025 Barcelona, Spain and and Universitat Autnoma de Cerdanyola del Valls, Spain Barcelona, 08193 Bellaterra |
| SynGO annotation ID: | 2395 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |