| Annotated protein: | Homer protein homolog 1 (Homer-1) (VASP/Ena-related gene up-regulated during seizure and LTP 1) (Vesl-1). Gene symbol: HOMER1. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q9Z2Y3-5 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ HOMER1 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic cytosol (GO:0099524) |
| Annotated paper: | Tao-Cheng JH, et al. "Homer is concentrated at the postsynaptic density and does not redistribute after acute synaptic stimulation" Neuroscience. 2014 Apr 25;266:80-90 PMID:24530450 |
| Figure(s): | Figures 1, 2 |
| Annotation description: | Homer1a is a short Homer1 isoform, upregulated in response to neuronal activity. This PMID demonstrates that Homer1a is cytosplasmic, and unlike the long isoforms, is NOT concentrated at the PSD. This was shown by biochemical fractionation (Fig1) and immuno-EM (Fig2) 9/3/2017 Pim: - Important statement by the authors on Fig.2 "In contrast, Homer 1a label was dispersed in neuronal somal and **dendritic cytoplasm** with no affiliation with PSD" (emphasis added). Moreover, Fig.1 also show presence of homer1a in synapse preparation. Together these observations are used to justify the "postsynaptic" element in the selected SynGO term. -Brain area that is featured in Fig.2 is unclear. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Biochemical fractionation (generic) |
| Annotator(s): | Harold MacGillavry (ORCID:0000-0002-6153-3586) Casper Hoogenraad (ORCID:0000-0002-2666-0758) |
| Lab: | Department of Biology, Faculty of Science, Utrecht University, 3584 CH Utrecht, The Netherlands |
| SynGO annotation ID: | 239 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |