| Annotated protein: | Dysbindin (Biogenesis of lysosome-related organelles complex 1 subunit 8) (BLOC-1 subunit 8) (Dysbindin-1) (Dystrobrevin-binding protein 1) (Hermansky-Pudlak syndrome 7 protein homolog) (HPS7 protein homolog). Gene symbol: DTNBP1. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q91WZ8 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ DTNBP1 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of synaptic vesicle exocytosis (GO:2000300) |
| Synapse type(s): | hippocampus, glutamatergic Schaffer collateral synapse (CA3->CA1) |
| Annotated paper: | Chen XW, et al. "DTNBP1, a schizophrenia susceptibility gene, affects kinetics of transmitter release" J Cell Biol. 2008 Jun 2;181(5):791-801 PMID:18504299 |
| Figure(s): | Fig 1, 2, 4, 5 |
| Annotation description: | This paper used the Sandy (sdy) mouse mutant that is defective in the Dysbindin-1 gene. Fig 1 shows that the amperomerty release kinetics (especially half-hight duration) of vesicles from sdy chromaffin cells are slower, and this is restored by reintroducing Dysbindin-1 in Fig 2, denatured Dysbindin does not show this effect. Fig 4 shows that these slow kinetics are also observed in hippocampal CA1 synapses, both on spontaneous events and evoked responses. Furthermore the RRP in chromaffin cells was also reduced, indicating that neurotransmitter secretion is affected in both models. |
| Evidence tracking, Biological System: | Intact tissue Non-neuronal tissue |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Whole-cell patch clamp Electrophysiology (generic) |
| Annotator(s): | Jan van Weering (ORCID:0000-0001-5259-4945) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | This paper shows that overexperssion of Dysbindin-1 enhances glutamate secretion (Fig 1) and knock-down of Dysbindin-1 inhibits glutamate secretion (Fig 3) from rat neuron cultures on slow time scales. @ PMID:15345706 |
| SynGO annotation ID: | 2383 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |