| Annotated protein: | Protocadherin-8 (Activity-regulated cadherin-like protein) (Arcadlin). Gene symbol: PCDH8. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: D3ZE55 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ PCDH8 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic membrane (GO:0099055) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Yasuda S, et al. "Activity-induced protocadherin arcadlin regulates dendritic spine number by triggering N-cadherin endocytosis via TAO2beta and p38 MAP kinases" Neuron. 2007 Nov 8;56(3):456-71 PMID:17988630 |
| Figure(s): | Fig.1 |
| Annotation description: | Fig.1: "Arcadlin Is Associated with N-Cadherin at Hippocampal Synaptic Puncta" Literal: The expression of arcadlin in cultures treated with IBMX and forskolin was confined to glutamate decarboxylase 65 (GAD6)-negative, non-GABAergic neurons (Figure 1E, arrow). In such neurons, arcadlin showed punctate distribution in dendrites (Figure 1F, arrow). The arcadlin puncta were not colocalized or apposed to the GAD6-puncta, which correspond to inhibitory axonal termini (Figure 1F, arrowhead). In contrast, the arcadlin-puncta colocalized with postsynaptic markers for excitatory synapses, such as PSD-95 and the NMDA receptor subunit NR1, as well as N-cadherin (Figure 1G). Arcadlin was expressed in developing axonal growth cones of young neurons, showing that arcadlin is also present in developing presynaptic membranes (Figure 1H, arrow). - Based on the known transmembrane topology of the protein, the "integral component of ... membrane" term was chosen. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2371 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |