| Annotated protein: | Heat shock cognate 71 kDa protein (EC 3.6.4.10) (Heat shock 70 kDa protein 8). Gene symbol: HSPA8. Taxonomy: Mus musculus (Mouse). Uniprot ID: P63017 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ HSPA8 |
| Ontology domain: | Biological Process |
| SynGO term: | presynaptic chaperone-mediated protein folding |
| Synapse type(s): | brain |
| Annotated paper: | Sharma M, et al. "CSPalpha promotes SNARE-complex assembly by chaperoning SNAP-25 during synaptic activity" Nat Cell Biol. 2011 Jan;13(1):30-9 PMID:21151134 |
| Figure(s): | Figure 5, 6 |
| Annotation description: | In this paper CSPalpha is shown to regulate the protein stability of SNAP25. This activity is mediated by Hsc70 and SGT. Fig.5: "CSPα binds to monomeric SNAP-25 through Hsc70." Fig.6: "The CSPα-Hsc70-SGT complex chaperones SNAP-25 and enhances its incorporation into SNARE complexes in vitro." 29/01/2018 Pim - Although the data in Fig.6 show the direct role of SGT-alpha (small glutamine-rich tetratricopeptide repeat protein alpha) in the chaperoning in a cell free system. The relevance for and occurence in vivo is suggested by the IP data in Fig.5. Here SNAP-25 IP resulted in selective CSPα, Hsc70 and SGT or SNARE proteins pulldown from brain homogenates. - Effects on synaptic transmission is not shown in these or other data since the CSP KO is lethal. A role in SV exocytosis is proposed by the authors. This may be a reasonable inference, but I decided not to annotate to term 'regulation of synaptic vesicle exocytosis'. |
| Evidence tracking, Biological System: | Intact tissue Cell-free system |
| Evidence tracking, Protein Targeting: | Over-expression |
| Evidence tracking, Experiment Assay: | Western blot IP + WB/MSMS |
| Annotator(s): | Noa Lipstein (ORCID:0000-0002-0755-5899) Cordelia Imig (ORCID:0000-0001-7351-8706) Vincent O'connor (ORCID:0000-0003-3185-5709) Nils Brose (ORCID:0000-0003-0938-8534) |
| Lab: | Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany |
| SynGO annotation ID: | 2359 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |