| Annotated protein: | Neuronal pentraxin-1 (NP1) (47 kDa taipoxin-binding protein) (Neuronal pentraxin I) (NP-I). Gene symbol: NPTX1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P47971 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ NPTX1 |
| Ontology domain: | Biological Process |
| SynGO term: | postsynaptic density assembly (GO:0097107) |
| Synapse type(s): | retina, glutamatergic |
| Annotated paper: | Farhy-Tselnicker I, et al. "Astrocyte-Secreted Glypican 4 Regulates Release of Neuronal Pentraxin 1 from Axons to Induce Functional Synapse Formation" Neuron. 2017 Oct 11;96(2):428-445.e13 PMID:29024665 |
| Figure(s): | Fig.1, 2 |
| Annotation description: | Fig.1: "Gpc4 Upregulates Release of the AMPAR Clustering Factor NP1 from RGC Neurons" Fig.2: "NP1-GluA1 Interaction Is Necessary for Gpc4 to Induce Structural Synapse Formation" NP1 is a protein that is know to bind and cluster AMPARs. This paper shows release of NP1 from the presynapses of retinal neurons by the glial factor Glypican 4, which in turn results in clustering of AMPARs. I have annotated to the site of action of NP1 (synaptic cleft) rather than the localization of storage (presynapse). The synaptic cleft is not shown directly but derived from the co-localization with AMPARs and the signal peptide of the NP1 protein. Literal; "Treating RGCs with astrocyte conditioned media (ACM) or purified soluble Gpc4 for 6 days increased the amount of NP1 accumulated on the surface of neuronal processes by 1.54-fold ± 0.16-fold and 1.84-fold ± 0.22-fold for ACM and Gpc4, respectively (Figures 1C and 1D). The same results were obtained using two different antibodies against NP1 (Figures S1C-S1F)." Literal: "These data demonstrate that soluble Gpc4 regulates synaptic recruitment of GluA1 and synaptogenesis by increasing the release of NP1, which binds GluA1 AMPARs to induce synapse formation." |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | RNAi / shRNA Antagonist / agonist Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2355 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |