| Annotated protein: | Protein shisa-6 (Cystine-knot AMPAR modulating protein of 52 kDa) (CKAMP52) (Shisa family member 6). Gene symbol: SHISA6. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q3UH99 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ SHISA6 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynaptic neurotransmitter receptor activity (GO:0098962) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Klaassen RV, et al. "Shisa6 traps AMPA receptors at postsynaptic sites and prevents their desensitization during synaptic activity" Nat Commun. 2016 Mar 2;7:10682 PMID:26931375 |
| Figure(s): | fig 5, 6 |
| Annotation description: | "Figure 5: Shisa6 decreases AMPAR deactivation rate and desensitization rate, enhances the steady-state current and slows recovery from desensitization." "Figure 6: Shisa6 prolongs synaptic AMPAR currents and reduces synaptic depression." "We identified Shisa6 as an intrinsic auxiliary subunit of AMPAR complexes in the mammalian brain with unique characteristics compared with the CKAMP44/Shisa9 member of this family (Supplementary Table 3). Physical association of Shisa6 with the pore-forming GluA proteins modulates receptor properties by slowing synaptic AMPAR current activation and desensitization. Shisa6 traps AMPARs at the postsynapse in vivo, slows desensitization kinetics and favours a sustained open state on prolonged activation. Together, these processes reduce short-term synaptic depression." |
| Evidence tracking, Biological System: | Intact tissue Non-neuronal tissue |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) Over-expression |
| Evidence tracking, Experiment Assay: | Whole-cell patch clamp |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2350 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |