| Annotated protein: | Carnitine O-palmitoyltransferase 1, brain isoform (CPT1-B) (EC 2.3.1.21) (CPT IC) (Carnitine O-palmitoyltransferase I, brain isoform) (CPTI-B) (Carnitine palmitoyltransferase 1C). Gene symbol: CPT1C. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q8BGD5 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CPT1C |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynaptic membrane neurotransmitter receptor levels (GO:0099072) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Fado R, et al. "Novel Regulation of the Synthesis of alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid (AMPA) Receptor Subunit GluA1 by Carnitine Palmitoyltransferase 1C (CPT1C) in the Hippocampus" J Biol Chem. 2015 Oct 16;290(42):25548-60 PMID:26338711 |
| Figure(s): | Figure 2A-C, 3, 4A-D, 4I-J, 5C-F, 6 |
| Annotation description: | CPT1C regulates AMPAR protein expression and, in turn, synaptic abundance of GluA1- and GluA2-AMPARs. mEPSC amplitude is decreased in CPT1C KO hippocampal neurons compared to WT (Fig. 2A-C). mEPSC frequency is unchanged. Immunostaining further showed a decrease in synaptic surface GluA1 and GluA2-AMPARs (Fig. 3A-C). Decreased GluA1 and GluA2 protein expression in hippocampal tissue extracts and cultured neurons from CPT1C KO animals compared to WT (Figure 4-5). Expression of other synaptic proteins was unaffected (Fig. 4C-D, 5E-F). Overexpression of exogenous CPT1C in KO neurons was sufficient to rescue GluA1 and GluA2 protein expression and significantly increase AMPAR protein levels in WT neurons (Fig. 4I-J). CPT1C regulates AMPAR protein levels post-transcriptionally. There was no significant difference in mRNA levels of AMPARs (Gria1, Gria2) isolated from WT and CPT1C KO cultured hippocampal neurons or hippocampal tissue (Figure 6). 12/2/2018 Pim - The authors show an increased level of AMPARs in the postsynaptic density membrane (term 'regulation of neurotransmitter receptor localization to postsynaptic specialization membrane (GO:0098696)'). Because the function of CPT1C; Carnitine palmitoyltransferase 1C is believed to be upstream of membrane insertion, I have approved of the less specific term 'regulation of postsynaptic membrane neurotransmitter receptor levels (GO:0099072)' selected by the expert. |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) Over-expression Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Whole-cell patch clamp Western blot |
| Annotator(s): | Hana Goldschmidt (ORCID:0000-0002-5676-366X) Richard Huganir (ORCID:0000-0001-9783-5183) |
| Lab: | Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA and Kavli Neuroscience Discovery Institute, Johns Hopkins University, Baltimore, MD 21205, USA |
| Additional literature: | AMPAR interacting protein CPT1C enhances surface expression of GluA1-containing receptor; Fig 5d-f overexpression of GFP-CPT1C enhances suface abundance of GluA1-AMPARs DIV 10 ctx neurons; GluA1 surface; enhancement of surGluA1 depends on palmitolylation state of GluA1 at C585 (mut to S = no enhancement of sGluA1 with CPT1C); does not alter AMPAR channel properties-role restricted to AMPAR trafficking; unlike other auxilliary subunits @ PMID:25698923 |
| SynGO annotation ID: | 2346 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |