| Annotated protein: | Protein shisa-7 (Cystine-knot AMPAR modulating protein of 59 kDa) (CKAMP59) (GABA(A) receptor auxiliary subunit Shisa7) (Shisa family member 7). Gene symbol: SHISA7. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q8C3Q5 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ SHISA7 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic specialization membrane (GO:0099060) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Schmitz LJM, et al. "The AMPA receptor-associated protein Shisa7 regulates hippocampal synaptic function and contextual memory" Elife. 2017 Dec 4;6:e24192 PMID:29199957 |
| Figure(s): | fig 1c,d,e |
| Annotation description: | Figure 1 c) immunofluorescence microscopy shows Shisa7 co-localization with Homer1 (marker protein of Glut postsynapses.) d) biochemical fractionation shows Shisa7 is enriched in PSD e) Precipitation of Flag-Shisa7 from HEK293 cells using a Flag antibody shows that upon co-expression it binds directly to homomeric GluA1, GluA2 and GluA3 receptors "integral component" based on the protein's transmembrane domain |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons Non-neuronal tissue |
| Evidence tracking, Protein Targeting: | Over-expression Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Western blot Biochemical fractionation (generic) IP + WB/MSMS |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2344 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |