| Annotated protein: | Protein shisa-6 (Cystine-knot AMPAR modulating protein of 52 kDa) (CKAMP52) (Shisa family member 6). Gene symbol: SHISA6. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q3UH99 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ SHISA6 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic density membrane (GO:0099061) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Klaassen RV, et al. "Shisa6 traps AMPA receptors at postsynaptic sites and prevents their desensitization during synaptic activity" Nat Commun. 2016 Mar 2;7:10682 PMID:26931375 |
| Figure(s): | fig 2, 3 |
| Annotation description: | Figure 2 a) triple-immunofluorescence staining of cultured Shisa6 KO for Flag-Shisa6, Gria2, Dlg4 shows Shisa6 co-localizes with AMPARs and PSD-95 at postsynaptic sites d) biochemical fractionation shows Shisa6 is enriched in PSD e,f) IP+WB shows co-immunoprecipitation of AMPAR and Shisa6 Figure 3 further supportes postsynaptic localization by demonstrating Shisa6-Dlg4 interaction. "integral component" based on the protein's transmembrane domain |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Western blot Biochemical fractionation (generic) FRET |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2342 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |