| Annotated protein: | Nectin-3 (Nectin cell adhesion molecule 3) (Poliovirus receptor-related protein 3) (CD antigen CD113). Gene symbol: NECTIN3. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q9JLB9 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ NECTIN3 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic density membrane (GO:0099061) |
| Synapse type(s): | Mossy fiber synapse (DG->CA3) |
| Annotated paper: | Mizoguchi A, et al. "Nectin: an adhesion molecule involved in formation of synapses" J Cell Biol. 2002 Feb 4;156(3):555-65 PMID:11827984 |
| Figure(s): | fig 2., fig 3. |
| Annotation description: | NECTIN3 was seen at the postsynaptic sides of puncta adherentia junctions between the mossy fiber terminals and dendrites of pyramidal cells. NECTIN3 was enriched in postsynaptic density fractions. 2/1/2018 Pim - Literal: "Immunoelectron microscopic analysis revealed that ~90% of the immunogold particles detected with the anti-nectin-1 and anti-nectin-3 Abs that were raised against the cytoplasmic domains of nectin-1α and -3α, respectively, were concentrated in an asymmetrical manner on the plasma membranes of the mossy fiber terminals (presynaptic side) and dendrites (postsynaptic side) of pyramidal cells, respectively, at puncta adherentia junctions (Fig. 2, A and B)." - Based on the known transmembrane topology of the protein, the "integral component of ... membrane" term was chosen. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Western blot Biochemical fractionation (generic) |
| Annotator(s): | Hwajin Jung (ORCID:0000-0001-6194-9648) Eunjoon Kim (ORCID:0000-0001-5518-6584) |
| Lab: | Center for Synaptic Brain Dysfunctions, Institute for Basic Science (IBS) and Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, South Korea |
| SynGO annotation ID: | 2323 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |