| Annotated protein: | Muscarinic acetylcholine receptor M2. Gene symbol: CHRM2. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q9ERZ4 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CHRM2 |
| Ontology domain: | Biological Process |
| SynGO term: | presynaptic modulation of chemical synaptic transmission (GO:0099171) |
| Synapse type(s): | hippocampus, acetylcholinergic neocortex, acetylcholinergic striatum, acetylcholinergic |
| Annotated paper: | Zhang W, et al. "Characterization of central inhibitory muscarinic autoreceptors by the use of muscarinic acetylcholine receptor knock-out mice" J Neurosci. 2002 Mar 1;22(5):1709-17 PMID:11880500 |
| Figure(s): | 1B, 2 and 3 |
| Annotation description: | The paper looks at muscarinic receptor modulation of the K+ evoked release of radiolabelled Acetylcholine (Figure 1B) , pre-incubated in brain slices, by generic muscarinic receptor agonist oxotremorine . The profound inhibition mediated by this agonist in hippocampal (Figure 2) and Cortical (Figure 3) brain slices slices from wild type mice is compared with those from mChR2 knockout mice. 18/01/2018 Pim - The ACh-release experiments are on slices and do not provide a direct synaptic context. The synaptic context is provided by the localization studies in Fig.5+6 that show co-localization of CHRM2 and the vesicular ACh transporter (only striatum shown). |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) Antagonist / agonist |
| Evidence tracking, Experiment Assay: | Radioimmunoassay |
| Annotator(s): | Noa Lipstein (ORCID:0000-0002-0755-5899) Cordelia Imig (ORCID:0000-0001-7351-8706) Vincent O'connor (ORCID:0000-0003-3185-5709) Nils Brose (ORCID:0000-0003-0938-8534) |
| Lab: | Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany |
| SynGO annotation ID: | 2308 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |