| Annotated protein: | Drebrin (Developmentally-regulated brain protein). Gene symbol: DBN1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q07266 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ DBN1 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic actin cytoskeleton (GO:0098871) |
| Synapse type(s): | neocortex, glutamatergic |
| Annotated paper: | Hayashi K, et al. "Modulatory role of drebrin on the cytoskeleton within dendritic spines in the rat cerebral cortex" J Neurosci. 1996 Nov 15;16(22):7161-70 PMID:8929425 |
| Figure(s): | Figures 3-8 |
| Annotation description: | This paper demonstrates localization of drebrin A in dendritic spines in the cerebral cortex using confocal and electron microscopy (figures 3,4, 5). This paper also presents data that Drebrin A co-sediments with other actin-binding proteins in the insoluble fraction (figure 6). And, immuno-EM labeling on synaptosomes showed Drebrin A labeling on filamentous materials that were associated with postsynaptic densities (figure 7). Finally, biochemical evidence is provided that drebrin interacts with actin and the actin-binding proteins gelsolin and myosin II (figure 8). Together these data indicate that Drebrin A is bound to the postsynaptic actin cytoskeleton. |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Confocal Western blot Biochemical fractionation (generic) Protein-protein interaction (generic) |
| Annotator(s): | Harold MacGillavry (ORCID:0000-0002-6153-3586) Casper Hoogenraad (ORCID:0000-0002-2666-0758) |
| Lab: | Department of Biology, Faculty of Science, Utrecht University, 3584 CH Utrecht, The Netherlands |
| SynGO annotation ID: | 229 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |