| Annotated protein: | Voltage-dependent L-type calcium channel subunit beta-1 (CAB1) (Calcium channel voltage-dependent subunit beta 1). Gene symbol: CACNB1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P54283 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CACNB1 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynapse (GO:0098794) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Obermair GJ, et al. "Reciprocal interactions regulate targeting of calcium channel beta subunits and membrane expression of alpha1 subunits in cultured hippocampal neurons" J Biol Chem. 2010 Feb 19;285(8):5776-91 PMID:19996312 |
| Figure(s): | Fig 5 |
| Annotation description: | Primary hippocampal neurons were fixed and stained with mouse monoclonal anti-beta1 antibody. anti-beta1 staining could be detected in the soma and dendrites (Fig. 1B). The staining appeared punctate along dendrites (Fig. 1C). Higher magnification micrographs of dendritic segments (Fig. 1B, lower panel) showed a punctate staining pattern of all isoforms of the beta subunit along the dendritic shaft (arrows) and adjacent to the shaft in positions typical for dendritic spines (open arrowheads). Overexpression and antibody staining of a V5 epitope tagged beta1 subunit in hippocampal primary culture neurons also showed a punctate and discretely clustered pattern along the dendritic shaft and and in the dendritic spines. Co-expression of soluble eGFP was used to identify dendrites and dendritic spines on a morphological basis (Fig. 2). Supplementary Fig 1. Specificity of anti-beta antibodies tested. Dysgenic myotubes were transfected with a V5 epitope containing version of the beta1 subunit. Cells were fixed and stained with antibodies against all 4 subunits but the antibodies did not label non-transfected neighboring cells, or cross-react with cells expressing any of the other beta isoforms (Suppl. Fig. 1C). 21/11/2017 Pim - literal: "Thus, β subunits display an isoform-specific expression in the axonal compartment in that β2, β3, and β4 isoforms are expressed throughout the axon, whereas β1a and β1b are largely excluded from the distal axon and small axonal branches." |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression |
| Evidence tracking, Experiment Assay: | Wide-field fluorescence |
| Annotator(s): | Ryan J. Farrell (ORCID:0000-0003-4022-8707) Ghazaleh Ashrafi (ORCID:0000-0001-7480-0826) Camila Pulido (ORCID:0000-0002-5648-066X) Jaime de Juan-Sanz (ORCID:0000-0002-1212-5623) Timothy Ryan (ORCID:0000-0003-2533-9548) |
| Lab: | Department of Biochemistry, Weill Cornell Medicine, New York, NY 10065, USA |
| SynGO annotation ID: | 2281 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |