| Annotated protein: | GRIP1-associated protein 1 (GRASP-1) [Cleaved into: GRASP-1 C-terminal chain (30kDa C-terminus form)]. Gene symbol: GRIPAP1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q9JHZ4 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ GRIPAP1 |
| Ontology domain: | Cellular Component |
| SynGO term: | extrinsic component of postsynaptic early endosome membrane (GO:0098998) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Hoogenraad CC, et al. "Neuron specific Rab4 effector GRASP-1 coordinates membrane specialization and maturation of recycling endosomes" PLoS Biol. 2010 Jan 19;8(1):e1000283 PMID:20098723 |
| Figure(s): | figure 1, 3, 4 |
| Annotation description: | this paper demonstrates that GRIPAP1(GRASP1) binds Rab4-GTP and is predominantly associated with Rab4-positive endosomes using EM and immunofluorescence co-localization of endogenous GRASP1 and Rab4, and live-imaging showed that GRASP1 moved together with mobile Rab4 endosomes in dendrites of hippocampal neurons (figure 3). These data together indicate that GRASP1 is localized to postsynaptic early endosome membrane by binding to Rab4 (extrinsic). 29/6/2017 Pim - There is synaptic context; "GRASP-1 is associated with punctate structures that occasionally extend beyond the dendritic shaft (arrowheads in Figure 3C), overlap with the synaptic markers PSD-95 (arrowheads in Figure 3D) and Bassoon (arrowheads in Figure 3E). |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Confocal Protein-protein interaction (generic) |
| Annotator(s): | Harold MacGillavry (ORCID:0000-0002-6153-3586) Casper Hoogenraad (ORCID:0000-0002-2666-0758) |
| Lab: | Department of Biology, Faculty of Science, Utrecht University, 3584 CH Utrecht, The Netherlands |
| SynGO annotation ID: | 228 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |