| Annotated protein: | Gamma-aminobutyric acid receptor subunit epsilon (GABA(A) receptor subunit epsilon). Gene symbol: GABRE. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q9ES14 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ GABRE |
| Ontology domain: | Biological Process |
| SynGO term: | transmitter-gated ion channel activity involved in regulation of postsynaptic membrane potential (GO:1904315) |
| Synapse type(s): | pons, GABAergic |
| Annotated paper: | Belujon P, et al. "Inhibitory transmission in locus coeruleus neurons expressing GABAA receptor epsilon subunit has a number of unique properties" J Neurophysiol. 2009 Oct;102(4):2312-25 PMID:19625540 |
| Figure(s): | Fig.1, 2, 5, 6, 7, 8 |
| Annotation description: | Authors use a combination of gene expression, protein localization, and comparison of intrinsic channel properties and sensitivity to a variety of GABAAR a(nta)gonists and allosteric modulators between fast GABA currents in LC neurons and heterologously expressed receptors in oocytes, to conclude that fast synaptic GABAA currents in locus coeruleus neurons are mediated by a GABAAR that contains the epsilon subunit: "Our results support the hypothesis that GABAA transmission in the LC relies on receptors with unique properties: 1) mRNA coding for epsilon, but not for any subunit in the gamma family, is expressed in the LC, and the epsilon subunit is found at the plasma membrane; 2) LC neurons in vivo display minimal sensitivity to the wide-spectrum benzodiazepine, flunitrazepam, a property conferred by the epsilon subunit on recombinant receptors; 3) sensitivity to benzodiazepine is differential because diazepam, another wide-spectrum benzodiazepine, is effective; and 4) the kinetic parameters of GABAergic transmission in the LC are unusually slow" |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antagonist / agonist |
| Evidence tracking, Experiment Assay: | Whole-cell patch clamp |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2267 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |