| Annotated protein: | Gamma-aminobutyric acid receptor subunit epsilon (GABA(A) receptor subunit epsilon). Gene symbol: GABRE. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q9ES14 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ GABRE |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic membrane (GO:0099055) |
| Synapse type(s): | pons, GABAergic |
| Annotated paper: | Belujon P, et al. "Inhibitory transmission in locus coeruleus neurons expressing GABAA receptor epsilon subunit has a number of unique properties" J Neurophysiol. 2009 Oct;102(4):2312-25 PMID:19625540 |
| Figure(s): | Fig.3 |
| Annotation description: | Fig.3: Labeling for GABRE can be seen restricted to the postsynaptic side intracellular and in the postsynaptic plasma membrane. No labeling is observed in the synaptic specializations, but the authors state this may be a result of the preembedding EM method. This notion is supported by IPCSs in LC neurons that shows hallmarks of involvement of GABAARs that contain the epsilon subunit: "Using in vivo extracellular and in vitro patch-clamp recordings in LC, we established that neuron firing rates, GABAactivated currents, and mIPSC charge were insensitive to the benzodiazepine flunitrazepam (FLU), in agreement with the characteristics of recombinant receptors including an epsilon subunit. Surprisingly, LC provided binding sites for benzodiazepines, and GABA-induced currents were potentiated by diazepam (DZP) in the micromolar range. A number of GABAAR ligands significantly potentiated GABA-induced currents, and zinc ions were only active at concentrations above 1 microM, further indicating that receptors were not composed of only alpha and beta subunits, but included an epsilonsubunit." However, I annotated only the direct experimental observation in Fig.3. - Based on the known transmembrane topology of the protein, the "integral component of ... membrane" term was chosen. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2266 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |