| Annotated protein: | Gamma-aminobutyric acid receptor subunit gamma-3 (GABA(A) receptor subunit gamma-3). Gene symbol: GABRG3. Taxonomy: Mus musculus (Mouse). Uniprot ID: P27681 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ GABRG3 |
| Ontology domain: | Biological Process |
| SynGO term: | transmitter-gated ion channel activity involved in regulation of postsynaptic membrane potential (GO:1904315) |
| Synapse type(s): | cerebral cortex, GABAergic |
| Annotated paper: | Baer K, et al. "Postsynaptic clustering of gamma-aminobutyric acid type A receptors by the gamma3 subunit in vivo" Proc Natl Acad Sci U S A. 1999 Oct 26;96(22):12860-5 PMID:10536013 |
| Figure(s): | Fig.2-4 |
| Annotation description: | Paper shows that GABARs in gamma2-/- animals are again targeted to synapses after ectopic overexpression of gamma3. This shows that gamma3 is part of a synaptic GABAR that control synaptic transmission (Fig.2, 3). That this role is performed by the endogenous gamma3 subunit is illlustrated in Fig.4 that shows that gamma3 is localized to postsynaptic sites in gamma2-/- animals: "Thus, GABAA receptor clustering can be mediated by both the endogenous and the ectopic γ3 subunit, a property best seen in the absence of the γ2 subunit." |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Whole-cell patch clamp |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2258 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |