| Annotated protein: | Gamma-aminobutyric acid receptor subunit gamma-2 (GABA(A) receptor subunit gamma-2). Gene symbol: GABRG2. Taxonomy: Mus musculus (Mouse). Uniprot ID: P22723 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ GABRG2 |
| Ontology domain: | Biological Process |
| SynGO term: | transmitter-gated ion channel activity involved in regulation of postsynaptic membrane potential (GO:1904315) |
| Synapse type(s): | cerebral cortex, GABAergic |
| Annotated paper: | Alldred MJ, et al. "Distinct gamma2 subunit domains mediate clustering and synaptic function of postsynaptic GABAA receptors and gephyrin" J Neurosci. 2005 Jan 19;25(3):594-603 PMID:15659595 |
| Figure(s): | Fig.9 |
| Annotation description: | Fig.9: "On transfection of γ2-/- neurons with the γ2 subunit (9E10γ-γ-γ) or with the 9E10α-γ-γ chimeric construct, the mIPSC frequency was restored to values similar to the wild-type control, consistent with restoration of function and postsynaptic localization of GABAA receptors. Consistent with normal immunoreactivity for GAD (Fig. 1B) and the vesicular inhibitory amino acid transporter (Schweizer e al., 2003) in γ2-/- neurons, efficient rescue of mIPSCs in transfected γ2-/- pyramidal cells confirmed that presynaptic function of GABAergic γ2-/- neurons was intact and that the functional deficit of GABAergic γ2-/- neurons was limited to the postsynaptic apparatus." |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) Over-expression |
| Evidence tracking, Experiment Assay: | Whole-cell patch clamp |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2256 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |