| Annotated protein: | Potassium voltage-gated channel subfamily D member 3 (Voltage-gated potassium channel subunit Kv4.3). Gene symbol: KCND3. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q9Z0V1 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ KCND3 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic specialization membrane (GO:0099060) |
| Synapse type(s): | cerebral cortex, GABAergic |
| Annotated paper: | Burkhalter A, et al. "Differential expression of I(A) channel subunits Kv4.2 and Kv4.3 in mouse visual cortical neurons and synapses" J Neurosci. 2006 Nov 22;26(47):12274-82 PMID:17122053 |
| Figure(s): | Figure 7, 8 |
| Annotation description: | Fig.7: "Electron micrographs showing that Kv4.3 is differentially expressed at putative inhibitory and excitatory synapses in mouse primary visual cortex. A, B, Kv4.3 immunoperoxidase labeling (dark reaction product marked by arrows) in thin dendrites is excluded from asymmetric synapses (AT1). The thick dendrite (D) does not express Kv4.3. C, D, Kv4.3 immunoperoxidase labeling (arrows) in postsynaptic membranes directly across Gray type 2 axon terminals (AT2) that contain flattened vesicles and form symmetric, putative inhibitory synapses with spine (Sp) and perikaryon (P) of a nonpyramidal cell [identified by infoldings of nuclear (Nu) membrane]" Fig.8: "Electron micrographs showing differential localization of Kv4.2 and Kv4.3 at GABAergic and non-GABAergic synapses in mouse primary visual cortex. A, Kv4.2 immunoperoxidase (arrows and arrowheads) expression in a GABA immunogold (black dots)-labeled section. Notice that Kv4.2 (arrows) is expressed in the spine (Sp) membrane but is excluded from the asymmetric synapse formed by a non-GABAergic Gray type 1 axon terminal (AT1). In contrast, Kv4.2 is expressed at a symmetric synapse formed by GABA immunogold-labeled Gray type 2 axon terminal. B, C, Postsynaptic localization of Kv4.3 immunoperoxidase labeling (arrows) at symmetric synapses formed by GABAergic (black dots) Gray type 2 axon terminals onto dendrite (D) and perikaryon (P). " Note: - Based on the known transmembrane topology of the protein, the "integral component of ... membrane" term was chosen. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Niels Cornelisse (ORCID:0000-0001-9425-2935) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2241 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |