| Annotated protein: | Potassium voltage-gated channel subfamily D member 2 (Voltage-gated potassium channel subunit Kv4.2). Gene symbol: KCND2. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q9Z0V2 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ KCND2 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic specialization membrane (GO:0099060) |
| Synapse type(s): | cerebral cortex, GABAergic |
| Annotated paper: | Burkhalter A, et al. "Differential expression of I(A) channel subunits Kv4.2 and Kv4.3 in mouse visual cortical neurons and synapses" J Neurosci. 2006 Nov 22;26(47):12274-82 PMID:17122053 |
| Figure(s): | Figure 6BC, 8A |
| Annotation description: | Fig.6: "Electron micrographs showing that Kv4.2 is differentially expressed at putative inhibitory and excitatory synapses in mouse primary visual cortex" - "A, Extrasynaptic localization of Kv4.2 immunoperoxidase labeling (arrows) at an asymmetric synapse onto a thin dendrite (D)" - "D, Extrasynaptic localization of Kv4.2 (arrows) at an asymmetric synapse onto GABA immunogold (black dots)-labeled dendrite. E, Synaptic localization of Kv4.2 at a symmetric synapse (AT2) onto a pyramidal cell soma (P). Notice that Kv4.2 is contained in the postsynaptic membrane associated with the synaptic cleft, " Fig.8: "Electron micrographs showing differential localization of Kv4.2 and Kv4.3 at GABAergic and non-GABAergic synapses in mouse primary visual cortex. A, Kv4.2 immunoperoxidase (arrows and arrowheads) expression in a GABA immunogold (black dots)-labeled section. Notice that Kv4.2 (arrows) is expressed in the spine (Sp) membrane but is excluded from the asymmetric synapse formed by a non-GABAergic Gray type 1 axon terminal (AT1). In contrast, Kv4.2 is expressed at a symmetric synapse formed by GABA immunogold-labeled Gray type 2 axon terminal. B, C, Postsynaptic localization of Kv4.3 immunoperoxidase labeling (arrows) at symmetric synapses formed by GABAergic (black dots) Gray type 2 axon terminals onto dendrite (D) and perikaryon (P). " Note: - Depending on the synapse KCND2 / Kv4.2 is present in the extrasynaptic or synaptic plasma membrane. - Based on the known transmembrane topology of the protein, the "integral component of ... membrane" term was chosen. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Niels Cornelisse (ORCID:0000-0001-9425-2935) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2240 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |