| Annotated protein: | Kalirin (EC 2.7.11.1) (Huntingtin-associated protein-interacting protein) (PAM COOH-terminal interactor protein 10) (P-CIP10) (Protein Duo) (Serine/threonine-protein kinase with Dbl- and pleckstrin homology domain). Gene symbol: KALRN. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P97924 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ KALRN |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic density (GO:0014069) |
| Synapse type(s): | cerebral cortex, glutamatergic |
| Annotated paper: | Xie Z, et al. "Kalirin-7 controls activity-dependent structural and functional plasticity of dendritic spines" Neuron. 2007 Nov 21;56(4):640-56 PMID:18031682 |
| Figure(s): | Fig.2, 3 |
| Annotation description: | Background: Authors show that kalirin protein locates to the dendritic spine to control actin dynamics. In a previous publication (PMID:10692441) the authors show that KALRN is enriched in the PSD fraction. Fig 2: "CaMKII interacts with and phosphorylates kalirin-7" "CaMKII coimmunoprecipitated with kalirin-7 and PSD-95 from rat forebrain homogenates (Figure 2A), suggesting that these proteins participate in the same multiprotein complex" Fig 3: Confocal microscopy of cortical pyramidal neurons show strong localisation of kalirin-7 at the tips of spines indicating specific localisation at the PSD. Fig 4: Competition with C-terminal Kalirin 7 peptide (competitor of interaction with PSD95) prevents Kalirin accumulation at dendritic spines |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Whole-cell patch clamp IP + WB/MSMS |
| Annotator(s): | John Chua (ORCID:0000-0002-5615-1014) |
| Lab: | Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117597, Neurobiology/Ageing Program, Life Sciences Institute, National University of Singapore and Institute of Molecular and Cell Biology, Agency for Science, Technology and Research (A*STAR), Singapore |
| Additional literature: | Paper by the same authors show that KALRN is enriched in the PSD fraction (Fig.5). @ PMID:10692441 |
| SynGO annotation ID: | 2238 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |