| Annotated protein: | Kalirin (EC 2.7.11.1) (Huntingtin-associated protein-interacting protein) (PAM COOH-terminal interactor protein 10) (P-CIP10) (Protein Duo) (Serine/threonine-protein kinase with Dbl- and pleckstrin homology domain). Gene symbol: KALRN. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P97924 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ KALRN |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of modification of postsynaptic actin cytoskeleton (GO:1905274) |
| Synapse type(s): | cerebral cortex, glutamatergic |
| Annotated paper: | Xie Z, et al. "Kalirin-7 controls activity-dependent structural and functional plasticity of dendritic spines" Neuron. 2007 Nov 21;56(4):640-56 PMID:18031682 |
| Figure(s): | Fig.6, 7 |
| Annotation description: | Background: authors show that kalirin is a CAMKII regulated protein that controls spine morphology and the retention of AMPARs in the dendritic spine (Fig.2). Kalirin is shown to act (in part) via Rac1 signaling needed for spine remodeling (Fig.1). Fig.5: "Kalirin-7 expression and spine localization are required for maintenance of mature spine morphology" Fig.6: "Kalirin-7 controls AMPA receptor maintenance in spines" - "Altogether, these data provide evidence that kalirin controls maintenance of AMPARs at the synapse and AMPAR-mediated synaptic transmission" - "These experiments strongly suggest that kalirin-7 promotes spine enlargement and GluR1 enrichment in spines by enhancing actin polymerization" Fig.7: "NMDAR-dependent spine delivery of AMPA receptors depends on CaMKII and kalirin spine targeting" Fig.8: "NMDAR-dependent spine enlargement and enhancement of AMPA receptor-mediated synaptic transmission depend on kalirin expression" Note: kalirin is shown to activate rac1 a known regulator of actin remodelling. In Fig.6 the authors use latrunculin an inhibitor of actin polymerization. Together this means that the effects on spine morphology and AMPAR localization are downstream of Rac1->actin This is captured in the final implementation: 'regulation of modification of postsynaptic actin cytoskeleton (GO:1905274)' regulates 'neurotransmitter receptor localization to postsynaptic specialization membrane (GO:0099645)' |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | RNAi / shRNA Over-expression |
| Evidence tracking, Experiment Assay: | Confocal Whole-cell patch clamp |
| Annotator(s): | John Chua (ORCID:0000-0002-5615-1014) |
| Lab: | Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117597, Neurobiology/Ageing Program, Life Sciences Institute, National University of Singapore and Institute of Molecular and Cell Biology, Agency for Science, Technology and Research (A*STAR), Singapore |
| SynGO annotation ID: | 2237 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |