| Annotated protein: | Monoacylglycerol lipase ABHD6 (EC 3.1.1.23) (2-arachidonoylglycerol hydrolase) (Abhydrolase domain-containing protein 6). Gene symbol: ABHD6. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q8R2Y0 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ ABHD6 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of retrograde trans-synaptic signaling by endocanabinoid (GO:0099178) |
| Synapse type(s): | neocortex, glutamatergic |
| Annotated paper: | Marrs WR, et al. "The serine hydrolase ABHD6 controls the accumulation and efficacy of 2-AG at cannabinoid receptors" Nat Neurosci. 2010 Aug;13(8):951-7 PMID:20657592 |
| Figure(s): | Figure 2, 6 |
| Annotation description: | Fig.2: "In homogenates prepared from neurons in primary culture, WWL70 inhibited ~50% of the total [3H]-2-AG hydrolysis (Fig. 2d)" In cortical layer 5 pyramidal neurons, inhibition of ABDH6 promotes induction of CB1-dependent long-term depression (eCB-LTD) at excitatory synapses using a subthreshold stimulation protocol (Figure 6). This effect is blocked by the CB1 receptor antagonist AM251 demonstrating the action of 2-AG on the presynaptic CB1 receptor (Figure 6). In cultured neurons, 2-AG hydrolysis under basal or stimulated conditions significantly decreases upon inhibition of ABDH6 activity using WWL70 (Figure 3, Supplementary Figure 4). 15/12/2017 Pim - The retrograde transsynaptic signaling aspect of the endocanabiniod signaling in the preparation featured in this paper has been shown in another paper (PMID:17684555): "Sub-threshold stimulation of pyramidal neurons in layer 2 of this brain area induces eCB-dependent LTD (eCB-LTD) in layer 5 when a non-specific inhibitor of 2-AG hydrolysis, URB602, is added." |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons Non-neuronal tissue |
| Evidence tracking, Protein Targeting: | Antagonist / agonist |
| Evidence tracking, Experiment Assay: | Field recordings radioisotope assay evidence |
| Annotator(s): | Hana Goldschmidt (ORCID:0000-0002-5676-366X) Richard Huganir (ORCID:0000-0001-9783-5183) |
| Lab: | Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA and Kavli Neuroscience Discovery Institute, Johns Hopkins University, Baltimore, MD 21205, USA |
| Additional literature: | Shows the retrograde transsynaptic signaling aspect of the endocanabiniod signaling in the preparation: "Sub-threshold stimulation of pyramidal neurons in layer 2 of this brain area induces eCB-dependent LTD (eCB-LTD) in layer 5 when a non-specific inhibitor of 2-AG hydrolysis, URB602, is added." @ PMID:17684555 |
| SynGO annotation ID: | 2207 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |