| Annotated protein: | Protein cornichon homolog 2 (CNIH-2) (Cornichon family AMPA receptor auxiliary protein 2) (Cornichon-like protein). Gene symbol: CNIH2. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q5BJU5 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CNIH2 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynaptic neurotransmitter receptor activity (GO:0098962) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Boudkkazi S, et al. "Cornichon2 dictates the time course of excitatory transmission at individual hippocampal synapses" Neuron. 2014 May 21;82(4):848-58 PMID:24853943 |
| Figure(s): | Figures 2B, 3C, 6, 7 |
| Annotation description: | CNIH2 regulates the gating of the postsynaptic AMPA receptors in the hippocampus. "CNIH2 Knockdown Speeds Mossy Cell EPSC Kinetics." Spontaneous EPSCs were recorded from hillar mossy cells transduced with CNIH2- or control shRNA. Knockdown of CNIH2 accelerated the decay kinetics and rise time of the EPSCs (Fig. 2B) compared to control cells without affecting the amplitude or frequency (Fig. 3C). Spontaneous EPSC kinetics measured in aspiny interneurons, which do not express CNIH2, were unaffected by CNIH2-shRNA (Fig. 5). Overexpression of CNIH2 in aspiny was sufficient to produce an EPSC phenotype similar to that observed in mossy cells (Fig. 6). Figure 7. "CNIH2 Knockdown Speeds the Decay of EPSCs in Individual MFB-Mossy Cell Synapses." EPSCs were recorded in mossy cells, transduced with either control or CNIH2-shRNA, in response to APs elicited in MFBs by a patch pipette. Fig. 7B "...knockdown of CNIH2 selectively affected the gating of the postsynaptic AMPARs." The EPSC amplitudes and paired pulse ratios were similar between CNIH2-knockdown and control, uninfected cells. Supplementary Figure S2: Validation of CNIH2-shRNA knockdown efficiency was demonstrated in hippocampal cultures by western blotting and high-resolution mass spectrometry (MS). Knockdown of CNIH2 had no significant effect on CNIH3, TARP g-8 or AMPAR subunits (GluA1-4) protein expression. 20/2/2018 Pim - Background: The effect of CNIH2 on intrinsic channel properties has been demonstrated in heterologous cells in earlier publications. The findings in PMID:24853943 now confirm similar obsevations in vivo. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | RNAi / shRNA |
| Evidence tracking, Experiment Assay: | Whole-cell patch clamp |
| Annotator(s): | Hana Goldschmidt (ORCID:0000-0002-5676-366X) Richard Huganir (ORCID:0000-0001-9783-5183) |
| Lab: | Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA and Kavli Neuroscience Discovery Institute, Johns Hopkins University, Baltimore, MD 21205, USA |
| Additional literature: | Shows he effect of CNIH2 on intrinsic channel properties has been demonstrated in heterologous cells. @ PMID:19265014 |
| SynGO annotation ID: | 2187 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |