| Annotated protein: | Gamma-aminobutyric acid receptor subunit alpha-6 (GABA(A) receptor subunit alpha-6). Gene symbol: GABRA6. Taxonomy: Mus musculus (Mouse). Uniprot ID: P16305 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ GABRA6 |
| Ontology domain: | Biological Process |
| SynGO term: | transmitter-gated ion channel activity involved in regulation of postsynaptic membrane potential (GO:1904315) |
| Synapse type(s): | Cerebellar Golgi cell to granule cell synapse |
| Annotated paper: | Accardi MV, et al. "alpha6-Containing GABAA Receptors Are the Principal Mediators of Inhibitory Synapse Strengthening by Insulin in Cerebellar Granule Cells" J Neurosci. 2015 Jul 1;35(26):9676-88 PMID:26134650 |
| Figure(s): | Fig.2-4 |
| Annotation description: | Fig.2: Authors show that furosemide can be used to distinguish alpha1 from alpha6 containing GABAArs. Fig.3+4: Application of furosemide prevents or reverses synaptic strengthening induced by mROS. Note: "Synaptic strengthening' is the effect of having more (alpha6-containing) GABAARs in the postsynaptic specialization that together generate more current and a resulting larger influence on the membrane potential. The application of the furosemide silences the newly aquired population of GABAARs at the synapse, masking the synaptic strengthening. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antagonist / agonist |
| Evidence tracking, Experiment Assay: | Whole-cell patch clamp |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2184 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |