| Annotated protein: | Gamma-aminobutyric acid receptor subunit alpha-6 (GABA(A) receptor subunit alpha-6). Gene symbol: GABRA6. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P30191 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ GABRA6 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic specialization membrane (GO:0099060) |
| Synapse type(s): | Mossy fiber synapse (DG->CA3) Cerebellar Golgi cell to granule cell synapse |
| Annotated paper: | Nusser Z, et al. "The alpha 6 subunit of the GABAA receptor is concentrated in both inhibitory and excitatory synapses on cerebellar granule cells" J Neurosci. 1996 Jan;16(1):103-14 PMID:8613776 |
| Figure(s): | Fig. 2-5 |
| Annotation description: | Comment: The GABRA6 expression is restricted to cerebellar granule cells and the lineage-related cochlear nucleus granule cells. Literal: - "An enrichment of immunoparticles for the alpha6 subunit was observed in synaptic junctions between Golgi cell terminals and granule cell dendrites using antibodies to both the C-terminal (Figs. 3, 5) and the N-terminal domains of the subunit (Figs. 3, 4). Particles could be present along the entire length of the synaptic specialization with an abrupt decrease in labeling outside the synapses (Figs. 3-5)." - "Surprisingly, immunoreactivity for the alpha6 subunit was als observed in asymmetrical synapses between the glutamatergic mossy fiber terminals and granule cell dendrites (Figs. 3-5). Regardless of whether antibodies to the C-terminal (Figs. 3, 5) or the N-terminal (Fig. 3) regions of the subunit were used for postembedding localization, an enrichment of immunoparticles was detected in many mossy synapses." |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2183 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |