| Annotated protein: | Proline-rich transmembrane protein 1 (Dispanin subfamily D member 1) (DSPD1) (Synapse differentiation-induced protein 4) (SynDIG4). Gene symbol: PRRT1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q6MG82 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ PRRT1 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic membrane (GO:0099055) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Kirk LM, et al. "Distribution of the SynDIG4/proline-rich transmembrane protein 1 in rat brain" J Comp Neurol. 2016 Aug 1;524(11):2266-80 PMID:26660156 |
| Figure(s): | Figure 8-9 |
| Annotation description: | PRRT1/SynDIG4 is a brain-specific type II transmembrane protein (extracellular C-terminus and intracellular N-terminus, Figure 2-3). Biochemical fractionation from adult rat brain tissue shows SynDIG4 is enriched in synaptosomes but not the postsynaptic density (Figure 8). Immunostaining in culture hippocampal neurons shows endogenous SynDIG4 protein is enriched at extrasynaptic sites along neuronal processes where is co-localizes with GluA1-AMPA receptors (Figure 9). Figure 1 demonstrates the specificity of the SynDIG4 antibody used in this study using COS cells and brain tissue. Table 1 describes conditions used for SynDIG4 antibodies and other antibodies used in this paper. |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Western blot Biochemical fractionation (generic) |
| Annotator(s): | Hana Goldschmidt (ORCID:0000-0002-5676-366X) Richard Huganir (ORCID:0000-0001-9783-5183) |
| Lab: | Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA and Kavli Neuroscience Discovery Institute, Johns Hopkins University, Baltimore, MD 21205, USA |
| SynGO annotation ID: | 2181 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |