| Annotated protein: | Synaptosomal-associated protein 23 (SNAP-23) (Vesicle-membrane fusion protein SNAP-23). Gene symbol: SNAP23. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: O70377 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ SNAP23 |
| Ontology domain: | Biological Process |
| SynGO term: | exocytic insertion of neurotransmitter receptor to postsynaptic membrane (GO:0098967) |
| Synapse type(s): | hippocampus, GABAergic |
| Annotated paper: | Gu Y, et al. "Differential vesicular sorting of AMPA and GABAA receptors" Proc Natl Acad Sci U S A. 2016 Feb 16;113(7):E922-31 PMID:26839408 |
| Figure(s): | Fig.2, 3 |
| Annotation description: | Fig.2: "Exocytic events of GluA2 and γ2S are constitutive events mediated by different SNAPs" SNAP23 is needed for constituitive insertion of GABAA receptor but not of AMPA receptor. Tested with fluorescence microscopy and phluorin-tagged receptor subunits. Used shRNA KD and rescue with OE of shRNA insensitive SNAP23. Fig.3: "Effects of of SNAP25 and SNAP23 on synaptic surface expression of GluA2 and γ2S and basal synaptic transmission" - Fig.3C/D: "Knockdown of SNAP23, but not SNAP25, significantly reduced synaptic surface levels of γ2, which colocalized with the inhibitory presynaptic marker VGAT (Fig. 3 C and D)" - Fig.3G/H: "Knockdown of SNAP23, but not SNAP25, significantly reduced GABAA-receptor mediated mIPSC amplitude (Fig. 3 G and H). These results are consistent with the specific effects of SNAP25 and SNAP23 on constitutive exocytosis and synaptic surface levels of GluA2 and γ2S, respectively. The SNAP25 or SNAP23-dependent exocytosis of AMPA or GABAA receptors significantly affects excitatory and inhibitory synaptic transmission in neurons at the basal state." |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | RNAi / shRNA |
| Evidence tracking, Experiment Assay: | Confocal Whole-cell patch clamp |
| Annotator(s): | Ruud Toonen (ORCID:0000-0002-9900-4233) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | PMID:20118925 |
| SynGO annotation ID: | 2162 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |