| Annotated protein: | Neuropilin and tolloid-like protein 1 (Brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor class A domains protein 1). Gene symbol: NETO1. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q8R4I7 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ NETO1 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic density membrane (GO:0099061) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Ng D, et al. "Neto1 is a novel CUB-domain NMDA receptor-interacting protein required for synaptic plasticity and learning" PLoS Biol. 2009 Feb 24;7(2):e41 PMID:19243221 |
| Figure(s): | Fig.2 |
| Annotation description: | Neto1 is an integral membrane protein which localizes to the postsynaptic density membrane in hippocampal neurons. Fig.2: "Neto1 is a PSD Protein Localized to Dendritic Spines and Interacts with PSD-95 through a C-Terminal PDZ Tripeptide" - Fig.2A: "Neto1 was prominently expressed in the crude synaptosomal (Figure 2A, lane S2) and PSD fractions, but was absent from the synaptic vesicle fraction (Figure 2A, lane LP2)." - Fig.2B/C/D: "We found that Neto1 immunostaining decorated MAP2 positive dendritic arbors and co-localized with that of PSD-95 (Figure 2B) and NR1 (Figure 2C). We also found that Neto1 co-localized with actin (Figure 2D), which is highly enriched in dendritic spines in the hippocampus [20]." - Fig.2E/F: "Altogether, these findings indicate that Neto1 associates with PSD-95 in brain synapses through the binding of its C-terminal tripeptide with the PDZ domains of PSD-95" Conclusion: "Together, these findings demonstrate that Neto1 is a component of the PSD of excitatory synapses." 12/12/2017 Pim - Based on the known transmembrane topology of the protein, the "integral component of ... membrane" term was chosen. |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons Non-neuronal tissue |
| Evidence tracking, Protein Targeting: | Over-expression Antagonist / agonist |
| Evidence tracking, Experiment Assay: | Confocal Western blot Biochemical fractionation (generic) IP + WB/MSMS Y2H |
| Annotator(s): | Hana Goldschmidt (ORCID:0000-0002-5676-366X) Richard Huganir (ORCID:0000-0001-9783-5183) |
| Lab: | Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA and Kavli Neuroscience Discovery Institute, Johns Hopkins University, Baltimore, MD 21205, USA |
| Additional literature: | Additional biochemical data on NETO1 localization. @ PMID:21734292 |
| SynGO annotation ID: | 2146 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |