| Annotated protein: | BDNF/NT-3 growth factors receptor (EC 2.7.10.1) (Neurotrophic tyrosine kinase receptor type 2) (TrkB tyrosine kinase) (Trk-B). Gene symbol: NTRK2. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q63604 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ NTRK2 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynapse (GO:0098794) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Sui WH, et al. "Myosin Va mediates BDNF-induced postendocytic recycling of full-length TrkB and its translocation into dendritic spines" J Cell Sci. 2015 Mar 15;128(6):1108-22 PMID:25632160 |
| Figure(s): | figure 8 |
| Annotation description: | Figure 8; "Myo5a mediates the postendocytic recycling of TrkB-FL and contributes to the BDNF-triggered translocation of TrkB-FL into dendritic spines and sustained downstream signaling. (A) Cultured hippocampal neurons (18-21 DIV) were transfected with GFP before the experiment and treated with monensin (10uM) or TAT-5aExon55-66 (1uM) for 30min, followed by BDNF (50ng/ml) stimulation for 60min. No BDNF was added for the control (CON) group. Then, neurons were fixed and permeabilized. TrkB-FL was stained with anti-TrkB (C-14) antibody and labeled with Alexa Fluor 594, as described in the Materials and Methods. Representative images of two types of TrkB-FL localization are shown: arrows indicate TrkB-FL localization in spines and arrowheads indicate TrkB-FL outside the spine. Magnifications are shown to the right. Scale bar: 5um. (B) Quantitative analysis of the percentage of TrkB-FL puncta in mature spines (mushroom-shaped) as a proportion of all TrkB-FL puncta for the cells shown in A. Graphs show mean±s.e.m. determined from the results of more than three independent experiments (n=90-100 spines from the secondary branches of dendrites of 8-10 neurons for each group per independent experiment). *P<0.05 versus CON group; #P<0.05 (χ2 test)." |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2135 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |