| Annotated protein: | TIAM Rac1 associated GEF 1. Gene symbol: TIAM1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: D3ZWV8 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ TIAM1 |
| Ontology domain: | Cellular Component |
| SynGO term: | extrinsic component of postsynaptic density membrane (GO:0099147) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Tolias KF, et al. "The Rac1-GEF Tiam1 couples the NMDA receptor to the activity-dependent development of dendritic arbors and spines" Neuron. 2005 Feb 17;45(4):525-38 PMID:15721239 |
| Figure(s): | figure 1 |
| Annotation description: | Figure 1E: "Postembedding immunogold EM labeling of Tiam1 at asymmetric axospinous synapses from adult rat hippocampus. Arrows point to gold particles coding for Tiam1 at the postsynaptic density (PSD) in dendritic spines" "To determine more rigorously whether Tiam1 concentrates at synapses, we performed immunogold electron microscopy (EM) on adult rat brain and found that, in the SR of CA1 hippocampus, Tiam1 is localized postsynaptically (Figure 1E). Quantitative analysis revealed that the highest density of Tiam1 labeling is at the synaptic junction, closely associated with the postsynaptic density (PSD) (Figure 1F)." 11/12/2017 Pim - The authors note: " The PHn-CC-Ex domain of Tiam1 has previously been shown to act in a specific dominant-negative manner (Kawauchi et al., 2003, Stam et al., 1997), most likely by binding to Tiam1-interacting proteins and thereby blocking the recruitment of endogenous Tiam1 to the plasma membrane where Tiam1 is active (Michiels et al., 1997)." In the immuno-EM pictures gold particles are shown very close to the PSD membrane. Interaction with the NMDA receptor that is localized in the PSD membrane. Tiam1 has membrane interacting PH-domains. Together I think it is safe to infer a plasma membrane localization for the Tiam1 protein. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2121 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |