| Annotated protein: | DnaJ (Hsp40) homolog, subfamily B, member 1 (Predicted), isoform CRA_b (Dnajb1 protein). Gene symbol: DNAJB1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: B0K030 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ DNAJB1 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic density (GO:0014069) |
| Synapse type(s): | cerebellum cerebral cortex forebrain |
| Annotated paper: | Suzuki T, et al. "Presence of molecular chaperones, heat shock cognate (Hsc) 70 and heat shock proteins (Hsp) 40, in the postsynaptic structures of rat brain" Brain Res. 1999 Jan 16;816(1):99-110 PMID:9878698 |
| Figure(s): | fig 4, 8 |
| Annotation description: | immuno-EM shows postsynaptic localization (Fig.8). additional evidence was added by biochemical fractionation+westernblot (Fig.4). "The synaptic localization of Hsp40 immunoreactivity in the cerebral cortex was also examined at the electron microscopic level. Postsynaptic structures but not presynaptic structures were stained (Fig. 8). PSDs were immunoreactive but rather weak compared to regions of subsynaptic web. Staining patterns of HSP70 and Hsp40 in the spine were similar." missing from Figure8 legend, but noted in Figure7 legend; "(a) and (b) Cerebral cortex, (c) and (d) cerebellum." HSP40 antibody source; PMID:1586970 |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Western blot Biochemical fractionation (generic) |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2118 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |