| Annotated protein: | Neuronal vesicle trafficking-associated protein 1 (M234) (Neuron-enriched endosomal protein of 21 kDa) (Neuron-specific protein family member 1) (p21). Gene symbol: NSG1. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q62092 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ NSG1 |
| Ontology domain: | Biological Process |
| SynGO term: | neurotransmitter receptor transport, endosome to postsynaptic membrane (GO:0098887) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Steiner P, et al. "Interactions between NEEP21, GRIP1 and GluR2 regulate sorting and recycling of the glutamate receptor subunit GluR2" EMBO J. 2005 Aug 17;24(16):2873-84 PMID:16037816 |
| Figure(s): | figure 4,7 |
| Annotation description: | the effects of the NEEP21 and GRIP1 interaction are tested via the ND overexpression of the interaction domains of the respective proteins (NEEP21 aa129164, GRIP1 aa8101112). NEEP21 aa129164 overexpression causes a reduction of Glur2 (but not Glur1) surface delivery after NDMA-induced AMPAR internalization (Fig.4). A similar observation was made with OE of ND GRIP1 aa8101112. OE of NEEP21 aa129164 does not lead to a general perturbatio of the endoctic pathways as assesed by recycling of transferrin (Fig.4G). The infusion of NEEP21 aa129164 is shown to change EPSC properties (changed current/voltage relationships) under basal conditions. The OE of NEEP21 aa129164 results in endosomal accumulation of AMPARs (Fig.7). note; referenced PMID:16037816 shows the M&M for (confocal) microscopy |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2112 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |