| Annotated protein: | TIAM Rac1 associated GEF 1. Gene symbol: TIAM1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: D3ZWV8 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ TIAM1 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of modification of postsynaptic actin cytoskeleton (GO:1905274) |
| Synapse type(s): | cerebral cortex, glutamatergic |
| Annotated paper: | Tolias KF, et al. "The Rac1-GEF Tiam1 couples the NMDA receptor to the activity-dependent development of dendritic arbors and spines" Neuron. 2005 Feb 17;45(4):525-38 PMID:15721239 |
| Figure(s): | Fig.3, 5, 6, 7 |
| Annotation description: | Fig.3: "NMDA Receptor Stimulation Induces Tiam1 Phosphorylation and Rac1 Activation" Fig.5: "Reduced Tiam1 Expression Results in Decreased Spine and Synapse Density" Fig.6: "Effect of Overexpression of Tiam1 Mutants on Spine Density" Fig.7: "Tiam1 Is Required for NMDA Receptor-Dependent Spine Growth and Intracellular Signaling" "Tiam1 antibodies were purchased (Santa Cruz) or generated by immunizing rabbits with GST-Tiam1 PDZ (aa 840-1025). Specificity of the Tiam1 antibodies was tested with peptide competition experiments (data not shown) and confirmed with RNAi (Figures 4A and 7B)." 11/12/2017 Pim - The effect of Tiam1 as a regulator of actin modifications is inferred (by me and the authors) based on the known role of Tiam1 as a Rac1 GEF, the known role of Rac1 in synaptic actin dynamics, the effect of Tiam1 perturbation on Rac1 activation in cultured neurons (Fig.3), and the effect of Tiam1 perturbation on dendritic spine parameters. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | RNAi / shRNA |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2105 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |