| Annotated protein: | Scribble planar cell polarity protein. Gene symbol: SCRIB. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: D3ZWS0 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ SCRIB |
| Ontology domain: | Biological Process |
| SynGO term: | neurotransmitter receptor transport, endosome to postsynaptic membrane (GO:0098887) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Piguel NH, et al. "Scribble1/AP2 complex coordinates NMDA receptor endocytic recycling" Cell Rep. 2014 Oct 23;9(2):712-27 PMID:25310985 |
| Figure(s): | Fig.3, S4, 5, 7 |
| Annotation description: | Fig.1: "Scrib1 Interacts and Colocalizes with GluN2 Subunits" Fig.3: "Modification of Scrib1 Levels Affects Surface Expression of Endogenous GluN2A and GluN2B-Containing Receptors" Scrib1 is shown to regulate postsynaptic membrane neurotransmitter receptor levels. Fig.S4: "Scrib1 Interacts with Tac-GluN2A and Tac-GluN2B Chimeras at the Plasma Membrane and in Endosomes of COS-7 Cells" Fig.5: "Scrib1 Promotes GluN2A Recycling and Inhibits GluN2B Recycling upon Stimulation in Neuron Culture" term: 'neurotransmitter receptor transport, endosome to postsynaptic membrane (GO:0098887)' Fig.7: "Scrib1 Regulates the Level of Synaptic NMDARs at Schaffer Collaterals CA1 Pyramidal Cells Synapse" Demonstrates in vivo (slice recording) relevance for the in vitro (cell culture) observations. The term 'recycling' is the ensome-to-plasma membrane transport of the NTR Overall: Experiments show that Scrib1 regulates the internalization and recycling of GluN2A and Glun2B in a differential manner depending on the activity state of the neuron. |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons Non-neuronal tissue |
| Evidence tracking, Protein Targeting: | RNAi / shRNA Over-expression |
| Evidence tracking, Experiment Assay: | Wide-field fluorescence Whole-cell patch clamp |
| Annotator(s): | Tony Cijsouw (ORCID:0000-0003-0912-1514) Thomas Biederer (ORCID:0000-0002-3670-7863) |
| Lab: | Department of Neuroscience, Tufts University School of Medicine, Boston, MA 02111, USA |
| SynGO annotation ID: | 2094 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |