| Annotated protein: | Voltage-dependent calcium channel gamma-5 subunit (Neuronal voltage-gated calcium channel gamma-5 subunit) (Transmembrane AMPAR regulatory protein gamma-5) (TARP gamma-5). Gene symbol: CACNG5. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q8VHW8 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CACNG5 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic membrane (GO:0099055) |
| Synapse type(s): | olfactory bulb, glutamatergic |
| Annotated paper: | Kato AS, et al. "AMPA receptor subunit-specific regulation by a distinct family of type II TARPs" Neuron. 2008 Sep 25;59(6):986-96 PMID:18817736 |
| Figure(s): | Figure 1, Supplementary Figure 1 |
| Annotation description: | TARP γ-5 is an integral membrane protein belonging to the type II family of TARPs. Biochemical fractionation shows TARP γ-5 localizes to synaptic membrane fractions, including the postsynaptic density, in the rat olfactory bulb (Figure 1B). Ectopic expression of TARP γ-5 in cultured cortical neurons shows TARP γ-5 is enriched in the soma and in puncta along neuronal processes which co-localize with the excitatory postsynaptic protein PSD-95 (Supplementary Figure 1). The TARP γ-5 antibody is not suitable for immunostaining (authors comment in paper). 12/2/2018 Pim - TARP-gamma 5 is shown to interact with AMPARs, co-localize with synaptic markers in cultured neurons and but does not seem to be enriched in the PSD fraction. Together this supports the term 'integral component of postsynaptic membrane (GO:0099055)' selected by the expert. - Based on the known transmembrane topology of the protein, the "integral component of ... membrane" term was chosen. |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Western blot Biochemical fractionation (generic) IP + WB/MSMS |
| Annotator(s): | Hana Goldschmidt (ORCID:0000-0002-5676-366X) Richard Huganir (ORCID:0000-0001-9783-5183) |
| Lab: | Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA and Kavli Neuroscience Discovery Institute, Johns Hopkins University, Baltimore, MD 21205, USA |
| SynGO annotation ID: | 2089 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |