| Annotated protein: | Clathrin light chain A (Lca). Gene symbol: CLTA. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P08081 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CLTA |
| Ontology domain: | Cellular Component |
| SynGO term: | synaptic vesicle membrane (GO:0030672) |
| Synapse type(s): | brain |
| Annotated paper: | Maycox PR, et al. "Clathrin-coated vesicles in nervous tissue are involved primarily in synaptic vesicle recycling" J Cell Biol. 1992 Sep;118(6):1379-88 PMID:1325974 |
| Figure(s): | figure 5 |
| Annotation description: | The authors purify synaptic fractions (synaptosomes) from rat brain. They then isolate clathrin-coated vesicles (CCVs) from the synaptic fractions. They demonstrate that the vast majority of CCVs purified in this way are from the presysnapse and contain synaptic vesicles as their major cargo. Western blot reveals the enrichment of clathrin light chain A in these fractions (upper band in figure 5). 6/12/2017 Pim - The authors show that the isolated vesicles have many hall mark components of SVs, but lack certain others. The CCVs therefore represent endocytosed/recycled SVs after release. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | none of the above |
| Evidence tracking, Experiment Assay: | Western blot Biochemical fractionation (generic) |
| Annotator(s): | Peter McPherson (ORCID:0000-0001-7806-5662) |
| Lab: | Department of Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, Montreal, QC H3A 2B4, Canada |
| SynGO annotation ID: | 2078 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |