| Annotated protein: | Gamma-aminobutyric acid receptor subunit alpha-3 (GABA(A) receptor subunit alpha-3). Gene symbol: GABRA3. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P20236 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ GABRA3 |
| Ontology domain: | Cellular Component |
| SynGO term: | synapse (GO:0045202) |
| Synapse type(s): | thalamus, GABAergic |
| Annotated paper: | Eyre MD, et al. "Setting the time course of inhibitory synaptic currents by mixing multiple GABA(A) receptor alpha subunit isoforms" J Neurosci. 2012 Apr 25;32(17):5853-67 PMID:22539847 |
| Figure(s): | Fig.4 |
| Annotation description: | This paper shows that mIPSCs in nucleus reticularis thalami (nRT) neurons are mediated by alpha3-containing GABA-A-Rs. The paper uses a combination of channel properties finger printing (channel decay kinetics), protein expression/localization to synapses, and a pharmacological agent (zolpidem) that in the given concentration and gene expression distinguishes alpha1- from alpha3-containing GABA-A-Rs. Only alpha1-containing GABA-A-Rs have sensitivity to zolpidem at the used concentration. Fig.4: Co-localization with gephyrin (marker protein of GABA/Glycine postsynaptic specialization) is shown. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2073 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |