| Annotated protein: | Glutamate receptor ionotropic, delta-2 (GluD2) (GluR delta-2 subunit). Gene symbol: GRID2. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q63226 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ GRID2 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic density membrane (GO:0099061) |
| Synapse type(s): | cerebellum, glutamatergic Parallel fibre (cerebellum -> Purkinje-cell) |
| Annotated paper: | Landsend AS, et al. "Differential localization of delta glutamate receptors in the rat cerebellum: coexpression with AMPA receptors in parallel fiber-spine synapses and absence from climbing fiber-spine synapses" J Neurosci. 1997 Jan 15;17(2):834-42 PMID:8987804 |
| Figure(s): | Figs.1, 2 and 5 |
| Annotation description: | Immunogold EM localization of GluR delta-2 subunit at the postsynaptic side of parallel fiber synapses onto dendritic spines of Purkinje cells. Note, the antibody also detects delta-1 subunits. 6/12/2017 Pim - The authors state that the antibody does not distinguish between GluD1 and GluD2. Howerver, for other reasons the authors conclude that the staining in the figures represent GluD2 protein: "The antibody used here recognizes the δ1 receptor as well as the δ2 receptor (Mayat et al., 1995). To assess the extent to which the δ1 receptor had contributed to the present pattern of immunolabeling, we processed the cerebellar sections together with hippocampal sections from the same animals. In situhybridization analyses have shown that, although the hippocampus does not contain detectable levels of δ2 subunit mRNA (Araki et al., 1993;Lomeli et al., 1993), it is the brain structure that is richest in δ1 mRNA (Lomeli et al., 1993). The very weak labeling obtained by the δ receptor antibody in synapses of coprocessed hippocampal sections suggests that the labeling in the cerebellum is attributable primarily to the presence of the δ2 receptor. This would agree with previous observations based on other approaches (Mayat et al., 1995). In the remaining Discussion, the labeling will be considered to reflect the δ2 receptor distribution." I allow annotation to GluD2 protein based on author and expert intent. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Daniela Dieterich (ORCID:0000-0002-9880-1214) Rainer Pielot (ORCID:0000-0002-9681-3318) Karl-Heinz Smalla (ORCID:0000-0002-0269-0311) Eckart Gundelfinger (ORCID:0000-0001-9377-7414) |
| Lab: | Leibniz Institute for Neurobiology (LIN), Institute of Pharmacology and Toxicology, Otto von Guericke University, Center for Behavioral Brain Sciences (CBBS), Magdeburg, Germany |
| Additional literature: | Immuno-EM localizing GluR delta1/2 immunoreactivity to postsynaptic spines of parallel fiber synapses in the cerebellum (Fig. 9) @ PMID:7891187 Figure 2: Immunogold EM shows the localisation of GRID2 in the postsynaptic density. @ PMID:18509461 |
| SynGO annotation ID: | 2071 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |