| Annotated protein: | Protein-serine O-palmitoleoyltransferase porcupine (mPORC) (EC 2.3.1.250). Gene symbol: PORCN. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q9JJJ7 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ PORCN |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynaptic membrane neurotransmitter receptor levels (GO:0099072) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Erlenhardt N, et al. "Porcupine Controls Hippocampal AMPAR Levels, Composition, and Synaptic Transmission" Cell Rep. 2016 Feb 2;14(4):782-794 PMID:26776514 |
| Figure(s): | Figures 3, 5, 6-7; Supplemental Figures 3 and 5 |
| Annotation description: | PORCN controls assembly and stability of hippocampal AMPARs at the level of the ER. shRNA-knockdown of PORCN in hippocampal neurons significantly reduced AMPAR-mediated currents (>50%, Figure 3A-B) and surface and total AMPAR levels (Figure 5A-C). Function of PORCN is independent of catalytic activity (Figure S3B). AMPAR complex components are reduced in extrasynaptic and synaptic fractions purified from PORCN KO forebrain tissue (Figure 6B-D). "PORCN knockout reduces basal synaptic transmission but not long-term potentiation" Figure 7 KO PORCN reduces Endo-H-sensitive and resistant pools of AMPARs in hippocampal neurons (TARPs and CNIH only effect EndoH-resistant, Supplemental Figure 5). 18/01/2018 Pim - In figures 7 it is shown that the amount of AMPARs in the PSD is reduced. The term 'regulation of neurotransmitter receptor localization to postsynaptic specialization membrane (GO:0098696)' was not awarded because in figure 6 it is shown that "PORCN knockout reduces synaptic and extrasynaptic AMPAR complexes". The effect on AMPAR levels in the PSD is downstream of the number of AMPARs in the membrane. |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) RNAi / shRNA Antibody (detection) |
| Evidence tracking, Experiment Assay: | Whole-cell patch clamp Field recordings Western blot Biochemical fractionation (generic) |
| Annotator(s): | Hana Goldschmidt (ORCID:0000-0002-5676-366X) Richard Huganir (ORCID:0000-0001-9783-5183) |
| Lab: | Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA and Kavli Neuroscience Discovery Institute, Johns Hopkins University, Baltimore, MD 21205, USA |
| Additional literature: | Figure 1A - membrane topology for PORCN, an ER-resident integral membrane protein @ PMID:24798332 Fig. 2 and Fig 5 shows palmitoyltransferase activity Porcupine on Wnt-1 occurs in ER (Drosophila) @ PMID:15166250 |
| SynGO annotation ID: | 2070 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |