| Annotated protein: | Gamma-aminobutyric acid receptor subunit alpha-1 (GABA(A) receptor subunit alpha-1). Gene symbol: GABRA1. Taxonomy: Mus musculus (Mouse). Uniprot ID: P62812 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ GABRA1 |
| Ontology domain: | Biological Process |
| SynGO term: | transmitter-gated ion channel activity involved in regulation of postsynaptic membrane potential (GO:1904315) |
| Synapse type(s): | hippocampus, GABAergic |
| Annotated paper: | Goldstein PA, et al. "Prolongation of hippocampal miniature inhibitory postsynaptic currents in mice lacking the GABA(A) receptor alpha1 subunit" J Neurophysiol. 2002 Dec;88(6):3208-17 PMID:12466441 |
| Figure(s): | Fig.1, 2, 3, 4, |
| Annotation description: | " To investigate the contribution of the α1 subunit to native GABAA-Rs, we analyzed miniature inhibitory postsynaptic currents (mIPSCs) in CA1 hippocampal pyramidal cells and interneurons from wild-type (WT) and α1 subunit knock-out (α1 KO) mice. mIPSCs recorded from interneurons and pyramidal cells obtained from α1 KO mice were detected less frequently, were smaller in amplitude, and decayed more slowly than mIPSCs recorded in neurons from WT mice. The effect of zolpidem was examined in view of its reported selectivity for receptors containing the α1 subunit. In interneurons and pyramidal cells from WT mice, zolpidem significantly increased mIPSC frequency, prolonged mIPSC decay, and increased mIPSC amplitude; those effects were diminished or absent in neurons from α1 KO mice. Nonstationary fluctuation analysis of mIPSCs indicated that the zolpidem-induced increase in mIPSC amplitude was associated with an increase in the number of open receptors rather than a change in the unitary conductance of individual channels. These data indicate that the α1 subunit is present at synapses on WT interneurons and pyramidal cells." Fig.1: "Characteristics of miniature inhibitory postsynaptic currents (mIPSCs) in interneurons." Fig.2: "Characteristics of mIPSCs in pyramidal cells. " Fig.3: "mIPSC reversal potential and block by bicuculline." Fig.4: "Zolpidem modulation of mIPSC properties in interneurons and pyramidal cells" |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) Antagonist / agonist |
| Evidence tracking, Experiment Assay: | Whole-cell patch clamp |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2054 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |