| Annotated protein: | Disks large homolog 3 (PSD-95/SAP90-related protein 1) (Synapse-associated protein 102) (SAP-102) (SAP102). Gene symbol: DLG3. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q62936 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ DLG3 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic density, intracellular component (GO:0099092) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Zheng CY, et al. "SAP102 is a highly mobile MAGUK in spines" J Neurosci. 2010 Mar 31;30(13):4757-66 PMID:20357126 |
| Figure(s): | Figure 1D |
| Annotation description: | SAP102 localizes to the postsynaptic density of excitatory synapses in CA1 neurons of the adult (P37) rat hippocampus (Figure 1D-E). Distinct from PSD-95, SAP102 is not palmitoylated nor anchored in the PSD-membrane, and can therefore localize to both the PSD and cytoplasm within dendritic spines. The median value for the location of SAP102 is 24.2nm from the postsynaptic membrane. SAP102 is more generally distributed in the cytoplasm between 50 and 250 nm from the postsynaptic membrane. Antibodies used in electron microscopy analyses were validated by western blot analysis of rat hippocampus and ectopic expressing in HEK cells (Figure 1) and immunogold labeling in CA1 stratum radiatum of the rat hippocampus over the course of development (Figure 4B). |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Hana Goldschmidt (ORCID:0000-0002-5676-366X) Richard Huganir (ORCID:0000-0001-9783-5183) |
| Lab: | Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA and Kavli Neuroscience Discovery Institute, Johns Hopkins University, Baltimore, MD 21205, USA |
| Additional literature: | Validation of SAP102 and PSD-95 antibodies used for immunogold labeling. @ PMID:10648730 |
| SynGO annotation ID: | 2052 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |