| Annotated protein: | Disks large homolog 4 (Postsynaptic density protein 95) (PSD-95) (Synapse-associated protein 90) (SAP-90) (SAP90). Gene symbol: DLG4. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P31016 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ DLG4 |
| Ontology domain: | Cellular Component |
| SynGO term: | anchored component of postsynaptic density membrane (GO:0099031) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Zheng CY, et al. "SAP102 is a highly mobile MAGUK in spines" J Neurosci. 2010 Mar 31;30(13):4757-66 PMID:20357126 |
| Figure(s): | Figure 1 |
| Annotation description: | PSD-95 is concentrated within the postsynaptic density of dendritic spines in CA1 neurons of the adult rat hippocampus (P37, Figure 1C-E). The median value for the location of PSD-95 is 13.5nm from the postsynaptic membrane. "anchored component" annotation is because PSD-95 requires palmitoylation for membrane association and synaptic localization. Antibodies used in electron microscopy analyses were validated by western blot analysis of rat hippocampus and ectopic expressing in HEK cells (Figure 1) and immunogold labeling in CA1 stratum radiatum of the rat hippocampus over the course of development (Figure 4B). |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Confocal |
| Annotator(s): | Hana Goldschmidt (ORCID:0000-0002-5676-366X) Richard Huganir (ORCID:0000-0001-9783-5183) |
| Lab: | Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA and Kavli Neuroscience Discovery Institute, Johns Hopkins University, Baltimore, MD 21205, USA |
| Additional literature: | Validation of antibodies used for immunogold labeling in Zheng CY et al (PMID 20357126). @ PMID:10648730 Figure 5 - PSD-95 immunogold labeling in SSC synapses of mouse hippocampus. @ PMID:27076426 |
| SynGO annotation ID: | 2051 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |