| Annotated protein: | Carnitine O-palmitoyltransferase 1, brain isoform (CPT1-B) (EC 2.3.1.21) (CPT IC) (Carnitine O-palmitoyltransferase I, brain isoform) (CPTI-B) (Carnitine palmitoyltransferase 1C). Gene symbol: CPT1C. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q8BGD5 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CPT1C |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic ER membrane |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Carrasco P, et al. "Ceramide levels regulated by carnitine palmitoyltransferase 1C control dendritic spine maturation and cognition" J Biol Chem. 2012 Jun 15;287(25):21224-32 PMID:22539351 |
| Figure(s): | Fig 1 |
| Annotation description: | CPT1C is a multipass membrane protein (uniprot Q8BGD5 or F1LN46, mouse and rat) expressed in pyramidal neurons (not astrocytes) of the hippocampus (Figure 1A). CPT1C is unique from the other two CPT1 isoforms due to its brain-specific expression and localization to the ER instead of the mitochondria. Distinct from the other two CPT1 isoforms (which localize to the outer mitochondrial membrane), the brain-specific CPT1C isoform localizes to the endoplasmic reticulum (ER) in cultured hippocampal neurons (Figure 1C-1D). Immunostaining of GFP-CPT1C in these cells shows CPT1C is present in the cell body and dendrites (both shaft and spines, Figure 1B). Several other studies in heterologous cells confirm the localization of CPT1C to the ER and not the mitochondria. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Western blot Biochemical fractionation (generic) |
| Annotator(s): | Hana Goldschmidt (ORCID:0000-0002-5676-366X) Richard Huganir (ORCID:0000-0001-9783-5183) |
| Lab: | Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA and Kavli Neuroscience Discovery Institute, Johns Hopkins University, Baltimore, MD 21205, USA |
| Additional literature: | Fig 2 - ER localization - immunostaining in heterologous cells; Fig 4- Biochemical fractionation from adult mouse brain; confirm presence in microsomal fraction and not mitochondria Plasmids generated in this paper are used in PMID: 22539351 @ PMID:18192268 Supplemental eFigure 3: human iPSC-derived motor neurons; CPT1C localizes to soma, dendrites and axons. Supplemental eFigure 5: ectopic expression of human HA-CPT1C in COS7 cells confirms localization to ER and not mitochondria @ PMID:25751282 |
| SynGO annotation ID: | 2048 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |